The effect of temperature on the surface tension of a lipid bi-layer in a biomembrane can be studied by measuring the surface tension at different temperatures and analyzing the fatty acid composition of the biomembrane. As temperature increases, the surface tension of the lipid bi-layer typically decreases. This is due to the increased thermal motion of the lipid molecules, which leads to a more disordered and fluid state of the membrane.To determine the changes in the structure and properties of the biomembrane, the following steps can be taken:1. Surface tension measurements: Surface tension can be measured using techniques such as the Wilhelmy plate method or the maximum bubble pressure method. By performing these measurements at different temperatures, the relationship between temperature and surface tension can be established.2. Fatty acid composition analysis: The fatty acid composition of the biomembrane can be analyzed using techniques such as gas chromatography or mass spectrometry. This will provide information about the types and proportions of fatty acids present in the lipid bi-layer.3. Correlating surface tension and fatty acid composition: By comparing the surface tension measurements and fatty acid composition analysis, the effect of temperature on the biomembrane's structure and properties can be determined. For example, an increase in the proportion of unsaturated fatty acids in the lipid bi-layer may lead to a decrease in surface tension at higher temperatures. This is because unsaturated fatty acids have kinks in their hydrocarbon chains, which disrupt the packing of the lipid molecules and increase fluidity.4. Investigating phase transitions: As temperature increases, lipid bi-layers may undergo phase transitions from a more ordered gel phase to a more disordered liquid-crystalline phase. This can be studied using techniques such as differential scanning calorimetry DSC or Fourier-transform infrared spectroscopy FTIR . These techniques can provide information about the temperature at which these phase transitions occur and how they affect the biomembrane's properties.In conclusion, the effect of temperature on the surface tension of a lipid bi-layer in a biomembrane can be studied by measuring surface tension at different temperatures and analyzing the fatty acid composition. The results can provide insights into the changes in the structure and properties of the biomembrane, such as increased fluidity and phase transitions.