The best analytical method for detecting and quantifying multiple pesticide residues in food samples is the QuEChERS Quick, Easy, Cheap, Effective, Rugged, and Safe method combined with liquid chromatography-tandem mass spectrometry LC-MS/MS or gas chromatography-tandem mass spectrometry GC-MS/MS .QuEChERS is a sample preparation method that involves extraction and cleanup of the food sample. It is widely used for the analysis of pesticide residues in various food matrices due to its simplicity, speed, and cost-effectiveness. The method involves the following steps:1. Homogenization of the food sample2. Extraction of the pesticide residues using an appropriate solvent, usually acetonitrile3. Addition of salts e.g., magnesium sulfate and sodium chloride to induce phase separation4. Cleanup of the extract using dispersive solid-phase extraction d-SPE with appropriate sorbents e.g., primary secondary amine, C18, or graphitized carbon black to remove matrix interferences5. Analysis of the cleaned extract using LC-MS/MS or GC-MS/MSLC-MS/MS and GC-MS/MS are powerful analytical techniques that allow for the separation, identification, and quantification of multiple pesticide residues in complex food matrices. These techniques offer high sensitivity, selectivity, and wide linear dynamic range, making them suitable for detecting trace levels of pesticide residues.To assess the potential health risks associated with consuming foods containing pesticide residues, the data obtained from the analysis can be compared to established maximum residue limits MRLs set by regulatory agencies, such as the European Food Safety Authority EFSA or the United States Environmental Protection Agency EPA . MRLs are the highest levels of pesticide residues legally allowed in food products and are set to ensure that the consumption of these products does not pose a risk to human health.If the levels of pesticide residues detected in the food samples are below the MRLs, it is considered safe for consumption. However, if the levels exceed the MRLs, further investigation and risk assessment may be required to determine the potential health risks associated with consuming these foods. Additionally, long-term exposure to pesticide residues, even at levels below the MRLs, should be considered in the overall risk assessment, as chronic exposure to certain pesticides may have cumulative effects on human health.