The type and concentration of substrate play a significant role in the rate of enzyme catalysis in the hydrolysis of sucrose by the sucrase enzyme. To understand this, let's first briefly discuss the enzyme-substrate interaction and the Michaelis-Menten model.Enzymes are biological catalysts that speed up chemical reactions by lowering the activation energy. They do this by binding to specific substrates and forming enzyme-substrate complexes. The sucrase enzyme specifically catalyzes the hydrolysis of sucrose into glucose and fructose.The Michaelis-Menten model is a widely accepted model that describes the kinetics of enzyme-catalyzed reactions. According to this model, the rate of an enzyme-catalyzed reaction V depends on the concentration of the substrate [S] and the enzyme's maximum rate Vmax and Michaelis constant Km .Now, let's discuss how the type and concentration of substrate affect the rate of enzyme catalysis in the hydrolysis of sucrose by sucrase enzyme:1. Type of substrate: Enzymes are highly specific for their substrates due to their unique active sites. The sucrase enzyme specifically catalyzes the hydrolysis of sucrose. If a different substrate is used, the sucrase enzyme will not be able to bind effectively, and the reaction rate will be significantly lower or nonexistent. Therefore, the type of substrate is crucial for the enzyme's ability to catalyze the reaction.2. Concentration of substrate: The concentration of substrate directly affects the rate of enzyme catalysis. As the substrate concentration increases, the rate of the reaction also increases because there are more substrate molecules available for the enzyme to bind. However, this increase in reaction rate is not linear. At low substrate concentrations, the reaction rate is directly proportional to the substrate concentration. But as the substrate concentration increases further, the reaction rate starts to plateau and approaches the enzyme's maximum rate Vmax . This is because the enzyme becomes saturated with substrate molecules, and all active sites are occupied. At this point, the reaction rate is limited by the enzyme concentration and its turnover rate.In summary, the type and concentration of substrate significantly affect the rate of enzyme catalysis in the hydrolysis of sucrose by the sucrase enzyme. The enzyme is specific for sucrose, and the reaction rate increases with increasing substrate concentration until it reaches a plateau when the enzyme becomes saturated.