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What is the net ATP yield in the process of glycolysis and gluconeogenesis in a mammalian liver cell? Show the complete reactions and indicate the enzymes involved in each step of the pathway.

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Glycolysis and gluconeogenesis are two metabolic pathways that occur in mammalian liver cells. Glycolysis is the process of breaking down glucose into pyruvate, while gluconeogenesis is the process of synthesizing glucose from non-carbohydrate precursors. Both pathways involve several enzymatic reactions, and the net ATP yield for the combined processes is zero because the ATP consumed in gluconeogenesis is equal to the ATP produced in glycolysis.Here are the reactions and enzymes involved in each step of glycolysis and gluconeogenesis:Glycolysis:1. Glucose + ATP  Glucose-6-phosphate + ADP  enzyme: hexokinase 2. Glucose-6-phosphate  Fructose-6-phosphate  enzyme: phosphoglucose isomerase 3. Fructose-6-phosphate + ATP  Fructose-1,6-bisphosphate + ADP  enzyme: phosphofructokinase 4. Fructose-1,6-bisphosphate  Dihydroxyacetone phosphate + Glyceraldehyde-3-phosphate  enzyme: aldolase 5. Dihydroxyacetone phosphate  Glyceraldehyde-3-phosphate  enzyme: triose phosphate isomerase 6. Glyceraldehyde-3-phosphate + NAD+ + Pi  1,3-bisphosphoglycerate + NADH + H+  enzyme: glyceraldehyde-3-phosphate dehydrogenase 7. 1,3-bisphosphoglycerate + ADP  3-phosphoglycerate + ATP  enzyme: phosphoglycerate kinase 8. 3-phosphoglycerate  2-phosphoglycerate  enzyme: phosphoglycerate mutase 9. 2-phosphoglycerate  Phosphoenolpyruvate + H2O  enzyme: enolase 10. Phosphoenolpyruvate + ADP  Pyruvate + ATP  enzyme: pyruvate kinase Gluconeogenesis:1. Pyruvate + ATP + HCO3-  Oxaloacetate + ADP + Pi  enzyme: pyruvate carboxylase 2. Oxaloacetate + GTP  Phosphoenolpyruvate + GDP + CO2  enzyme: phosphoenolpyruvate carboxykinase 3. Phosphoenolpyruvate  2-phosphoglycerate  reverse of glycolysis steps 9-3, using the same enzymes 4. Fructose-1,6-bisphosphate  Fructose-6-phosphate + Pi  enzyme: fructose-1,6-bisphosphatase 5. Fructose-6-phosphate  Glucose-6-phosphate  reverse of glycolysis step 2, using the same enzyme 6. Glucose-6-phosphate  Glucose + Pi  enzyme: glucose-6-phosphatase In glycolysis, there is a net production of 2 ATP molecules per glucose molecule. However, in gluconeogenesis, 6 ATP equivalents  4 ATP and 2 GTP  are consumed per glucose molecule synthesized. Therefore, the net ATP yield for the combined processes of glycolysis and gluconeogenesis in a mammalian liver cell is zero.
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