During a fasting state, the body's glucose levels decrease as it is not receiving any new source of glucose from food. In response to this, the pancreas reduces the secretion of insulin, a hormone that promotes glucose uptake and utilization by cells. Insulin plays a crucial role in regulating glucose levels in the body by facilitating the uptake of glucose into cells, particularly in muscle and adipose tissue. It also stimulates the conversion of glucose to glycogen in the liver and muscles for storage. When insulin levels are low during fasting, these processes are slowed down.Instead, the body starts to break down stored glycogen in the liver and muscles to release glucose back into the bloodstream, a process known as glycogenolysis. Additionally, the liver starts producing glucose from non-carbohydrate sources, such as amino acids and glycerol, through a process called gluconeogenesis.Furthermore, low insulin levels during fasting also stimulate the breakdown of stored fat lipolysis in adipose tissue, releasing fatty acids and glycerol into the bloodstream. The fatty acids can be used as an alternative energy source by most cells, while glycerol can be used for gluconeogenesis in the liver.In summary, during a fasting state, the effect of insulin on the regulation of glucose levels in the body is reduced. This allows the body to maintain blood glucose levels by breaking down stored glycogen, producing glucose through gluconeogenesis, and utilizing alternative energy sources such as fatty acids.