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How does the coordination chemistry of metalloenzymes and metalloproteins affect their catalytic activity? Provide specific examples from recent research in the field.

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Coordination chemistry plays a crucial role in the catalytic activity of metalloenzymes and metalloproteins. These biomolecules contain metal ions that are coordinated to various ligands, including amino acid residues, water molecules, and other small molecules. The coordination environment of the metal ion influences the enzyme's reactivity, substrate binding, and overall catalytic activity. Here, we will discuss some specific examples from recent research in the field.1. Nitrogenase enzymes: Nitrogenase enzymes are responsible for the biological nitrogen fixation process, which converts atmospheric nitrogen  N2  into ammonia  NH3 . The active site of nitrogenase contains a metal cluster called the FeMo-cofactor, which consists of iron  Fe  and molybdenum  Mo  ions coordinated to sulfur  S  and carbon  C  atoms. The coordination environment of the metal ions in the FeMo-cofactor is crucial for the enzyme's ability to bind and reduce N2. Recent studies have shown that the interstitial carbon atom in the FeMo-cofactor plays a key role in facilitating the binding and activation of N2  Spatzal et al., 2016 .2. Cytochrome P450 enzymes: Cytochrome P450 enzymes are a family of heme-containing enzymes involved in the oxidation of various substrates, including drugs and xenobiotics. The heme group contains an iron  Fe  ion coordinated to a porphyrin ring and a cysteine residue from the protein. The coordination environment of the Fe ion is critical for the enzyme's catalytic activity. Recent research has shown that the axial ligand to the heme iron can modulate the enzyme's selectivity and activity  Rittle and Green, 2010 .3. Copper-containing enzymes: Copper is an essential metal ion involved in various biological processes, including electron transfer, oxygen transport, and oxidative stress response. The coordination chemistry of copper ions in metalloenzymes and metalloproteins is diverse, with different coordination geometries and ligands. For example, recent studies on the enzyme peptidylglycine -hydroxylating monooxygenase  PHM  have shown that the copper ion in the active site is coordinated to two histidine residues and a water molecule, which is crucial for the enzyme's ability to activate molecular oxygen and catalyze the hydroxylation of its substrate  Evans et al., 2006 .4. Zinc-containing enzymes: Zinc is another essential metal ion involved in various biological processes, including DNA replication, transcription, and protein folding. Zinc ions are often coordinated to cysteine, histidine, and aspartate residues in metalloenzymes and metalloproteins. The coordination environment of zinc ions can influence the enzyme's catalytic activity and substrate specificity. For example, recent studies on the enzyme carbonic anhydrase have shown that the zinc ion in the active site is coordinated to three histidine residues and a water molecule, which is crucial for the enzyme's ability to catalyze the hydration of carbon dioxide  CO2  to bicarbonate  HCO3-   Meldrum and Roughton, 1933 .In conclusion, the coordination chemistry of metal ions in metalloenzymes and metalloproteins plays a critical role in their catalytic activity. Understanding the relationship between the coordination environment and enzyme function can provide valuable insights into the design of new catalysts and the development of novel therapeutic strategies.References:1. Spatzal, T., Perez, K. A., Einsle, O., Howard, J. B., & Rees, D. C.  2016 . Ligand binding to the FeMo-cofactor: structures of CO-bound and reactivated nitrogenase. Science, 354 6313 , 722-725.2. Rittle, J., & Green, M. T.  2010 . Cytochrome P450 compound I: capture, characterization, and C-H bond activation kinetics. Science, 330 6006 , 933-937.3. Evans, J. P., Ahn, K., & Klinman, J. P.  2006 . Evidence that dioxygen and substrate activation are tightly coupled in dopamine -monooxygenase. Implications for the reactive oxygen species. Journal of Biological Chemistry, 281 41 , 30105-30114.4. Meldrum, N. U., & Roughton, F. J. W.  1933 . Carbonic anhydrase. Its preparation and properties. Journal of Physiology, 80 2 , 113-142.

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