0 votes
47 views
ago in Coordination Chemistry by (620 points)
What is the coordination number and geometry of the metal center in the active site of the metalloenzyme carbonic anhydrase? Furthermore, what is the role of the metal center in the catalytic mechanism of the enzyme?

1 Answer

0 votes
ago by (250 points)
Carbonic anhydrase is a metalloenzyme that contains a zinc  Zn  ion in its active site. The coordination number of the zinc ion in the active site is 4. The geometry of the metal center is tetrahedral. The zinc ion is coordinated to three histidine residues from the protein and a water molecule or hydroxide ion.The role of the metal center in the catalytic mechanism of carbonic anhydrase is to facilitate the interconversion of carbon dioxide  CO2  and water  H2O  into bicarbonate  HCO3-  and a proton  H+ . The zinc ion in the active site acts as a Lewis acid, polarizing the water molecule or hydroxide ion bound to it. This increases the nucleophilicity of the oxygen atom, allowing it to attack the carbon atom of CO2 more effectively.The mechanism of carbonic anhydrase can be summarized in the following steps:1. The zinc-bound water molecule loses a proton to a nearby residue  usually a histidine , generating a zinc-bound hydroxide ion.2. The hydroxide ion attacks the carbon atom of CO2, forming a zinc-bound bicarbonate ion.3. The bicarbonate ion is released from the active site, and a new water molecule binds to the zinc ion, resetting the enzyme for another catalytic cycle.In summary, the coordination number of the metal center in carbonic anhydrase is 4, with a tetrahedral geometry. The metal center plays a crucial role in the enzyme's catalytic mechanism by polarizing the water molecule or hydroxide ion, facilitating the interconversion of CO2 and H2O into HCO3- and H+.

Related questions

Welcome to Sarvan Science Q&A, where you can ask questions and receive answers from other members of the community.
...