A suitable supramolecular assembly for drug delivery can be designed using amphiphilic block copolymers, which are composed of hydrophilic and hydrophobic segments. These copolymers can self-assemble into micelles, vesicles, or other nanostructures in an aqueous environment, driven by non-covalent interactions such as hydrophobic effects, hydrogen bonding, and van der Waals forces. The hydrophobic core of these structures can encapsulate hydrophobic drug molecules, while the hydrophilic shell ensures stability and biocompatibility in an aqueous environment.One example of such a supramolecular assembly is a polymeric micelle formed by amphiphilic block copolymers, such as poly ethylene glycol -block-poly lactic-co-glycolic acid PEG-b-PLGA . The PEG segment is hydrophilic, while the PLGA segment is hydrophobic. In an aqueous environment, the hydrophobic PLGA segments aggregate to form the core of the micelle, while the hydrophilic PEG segments form the outer shell.The self-assembly of these micelles can be achieved through a variety of techniques in physical chemistry, such as solvent evaporation, dialysis, or direct dissolution. In the solvent evaporation method, the amphiphilic block copolymer and the hydrophobic drug are dissolved in an organic solvent, which is then evaporated under reduced pressure. As the solvent evaporates, the concentration of the copolymer increases, and the hydrophobic segments begin to aggregate, forming micelles with the drug encapsulated in the core. The micelles can then be isolated by centrifugation or filtration.In the dialysis method, the amphiphilic block copolymer and the hydrophobic drug are dissolved in a water-miscible organic solvent, such as dimethyl sulfoxide DMSO or tetrahydrofuran THF . This solution is then placed in a dialysis bag with a molecular weight cut-off that retains the micelles but allows the solvent and any unencapsulated drug to pass through. The dialysis bag is immersed in an aqueous buffer, and the organic solvent diffuses out of the bag, leading to the self-assembly of micelles with the drug encapsulated in the core.In the direct dissolution method, the amphiphilic block copolymer is dissolved in water, followed by the addition of the hydrophobic drug. The drug molecules partition into the hydrophobic domains of the copolymer, leading to the self-assembly of micelles with the drug encapsulated in the core.These supramolecular assemblies can be used for drug delivery due to their ability to encapsulate hydrophobic drugs, protect them from degradation, and enhance their solubility and bioavailability. Additionally, the size, shape, and surface properties of these assemblies can be tuned by adjusting the composition and molecular weight of the block copolymers, allowing for targeted drug delivery and controlled release.