To determine the concentration of a specific compound in a given sample using liquid chromatography, you need to follow these steps:1. Prepare the sample: Extract the compound of interest from the sample matrix, if necessary, and dissolve it in a suitable solvent compatible with the liquid chromatography system.2. Prepare a calibration curve: Analyze a series of standard solutions containing known concentrations of the compound of interest. Inject each standard solution into the liquid chromatography system and record the peak area or peak height for each concentration. Plot the peak area or height versus the concentration of the standard solutions to create a calibration curve. Perform a linear regression analysis to obtain the equation of the line y = mx + b , where y is the peak area or height, x is the concentration, m is the slope, and b is the y-intercept.3. Analyze the sample: Inject the prepared sample into the liquid chromatography system and record the peak area or height corresponding to the compound of interest.4. Calculate the concentration: Use the equation of the calibration curve to calculate the concentration of the compound in the sample. Rearrange the equation to solve for x concentration : x = y - b / m. Substitute the sample's peak area or height y into the equation and solve for x.5. Adjust for dilution: If you diluted the sample during the extraction or sample preparation process, multiply the calculated concentration by the dilution factor to obtain the concentration of the compound in the original sample.Keep in mind that the accuracy and precision of the concentration determination depend on the quality of the calibration curve, the sensitivity and selectivity of the liquid chromatography system, and the proper execution of the sample preparation and analysis procedures.