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A chemistry student needs to determine the concentration of iron (III) ions in a water sample using spectrophotometry. The water sample contains an unknown amount of iron (III) ions that need to be quantified with a high degree of accuracy. The student has access to a spectrophotometer and an iron (III) ion standard solution t

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To determine the concentration of iron  III  ions in the water sample, the student should follow these steps:1. Prepare a series of standard solutions with known concentrations of iron  III  ions by diluting the iron  III  ion standard solution. For example, prepare five standard solutions with concentrations of 1, 2, 4, 6, and 8 ppm  parts per million .2. Measure the absorbance of each standard solution using the spectrophotometer at the appropriate wavelength for iron  III  ions  typically around 510 nm . Record the absorbance values.3. Plot a calibration curve by graphing the absorbance values  y-axis  against the known concentrations of iron  III  ions  x-axis . The graph should be a straight line, and the equation of the line can be determined using linear regression  y = mx + b, where m is the slope and b is the y-intercept .4. Measure the absorbance of the unknown water sample using the spectrophotometer at the same wavelength as the standard solutions.5. Use the equation of the calibration curve to calculate the concentration of iron  III  ions in the water sample. Plug the absorbance value of the unknown sample into the equation and solve for x  the concentration .For example, if the equation of the calibration curve is y = 0.5x + 0.1 and the absorbance of the unknown sample is 1.7, the calculation would be:1.7 = 0.5x + 0.11.6 = 0.5xx = 3.2 ppmSo, the concentration of iron  III  ions in the water sample would be 3.2 ppm.

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