enzymes
Enzymes Enzymes, catalysts that occur naturally in living organisms, are almost all protein molecules with typical molecular masses of 20,000100,000 amu. Some are homogeneous catalysts that react in aqueous solution within a cellular compartment of an organism. Others are heterogeneous catalysts embedded within the membranes that separate cells and cellular compartments from their surroundings. The reactant in an enzyme-catalyzed reaction is called a substrate. Because enzymes can increase reaction rates by enormous factors up to 10 17 times the uncatalyzed rate and tend to be very specific, typically producing only a single product in quantitative yield, they are the focus of active research. At the same time, enzymes are usually expensive to obtain, they often cease functioning at temperatures greater than 37C, have limited stability in solution, and have such high specificity that they are confined to turning one particular set of reactants into one particular product. This means that separate processes using different enzymes must be developed for chemically similar reactions, which is time-consuming and expensive. Thus far, enzymes have found only limited industrial applications, although they are used as ingredients in laundry detergents, contact lens cleaners, and meat tenderizers. The enzymes in these applications tend to be proteases, which are able to cleave the amide bonds that hold amino acids together in proteins. Meat tenderizers, for example, contain a protease called papain, which is isolated from papaya juice. It cleaves some of the long, fibrous protein molecules that make inexpensive cuts of beef tough, producing a piece of meat that is more tender. Some insects, like the bombadier beetle, carry an enzyme capable of catalyzing the decomposition of hydrogen peroxide to water Figure 14.28 "A Catalytic Defense Mechanism" . Enzyme inhibitors cause a decrease in the reaction rate of an enzyme-catalyzed reaction by binding to a specific portion of an enzyme and thus slowing or preventing a reaction from occurring. Irreversible inhibitors are therefore the equivalent of poisons in heterogeneous catalysis. One of the oldest and most Saylor URL: http://www. saylor. org/books.