mutations
A gene pool is the complete set of unique alleles in a species or population. Mutations create variation in the gene pool. Populations with a large gene pool are said to be genetically diverse and very robust. They are able to survive intense times of natural selection against certain phenotypes. During these times of selection, individuals with less favorable phenotypes resulting from deleterious alleles due to mutations may be selected against and removed from the population. Concurrently, the more favorable mutations that cause beneficial or advantageous phenotypes tend to accumulate in that population, resulting, over time, in evolution. In fact, without any change in the gene pool, without any new alleles added due to new mutations, evolution could not occur. Genetic change is the driving force of evolution. In fact, evolution can be genetically defined as the change allele frequencies over time.