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Vertebrates differ from invertebrates because they lack this?

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backbone or a spinal column

The first vertebrates evolved about 550 million years ago. The evolution of amphibians, reptiles, mammals, and birds occurred over the next 400 million years. The first vertebrates were ectotherms; endothermy evolved later. Modern fish, amphibians, and reptiles are ectotherms. Modern birds and mammals are endotherms.

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